OGT Antikörper (N-Term)

Produktnummer ABIN2783662

Produktdetails anti-OGT Antikörper

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Hund, Zebrafisch (Danio rerio), Meerschweinchen, Pferd, Kaninchen, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OGT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: ASSVGNVADS TEPTKRMLSF QGLAELAHRE YQAGDFEAAE RHCMQLWRQE

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against OGT. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human OGT

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1046 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-OGT Antikörper (ABIN2783662)

Taylor, Parker, Hazel, Soesanto, Fuller, Yazzie, McClain: "Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6050-7, (2008) (PubMed).

Details zu OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Andere Bezeichnung: OGT (OGT Produkte)

Hintergrund: OGT catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: FLJ23071, HRNT1, MGC22921, O-GLCNAC
Protein Interaction Partner: FBXW11, HUWE1, UBC, PSG1, PHC3, NUP62CL, TET2, SUMO1, NEDD8, WWOX, BAP1, P3H1, RPRD1B, PPME1, LAP3, DBNL, HSPBP1, GPN1, WDR4, ACTR2, TOM1L1, ZPR1, SURF2, LPP, AGFG1, ALAD, C14orf142, UBL4A, FOXO4, HCFC1, SMURF1, Hoxa1, Nfatc1, Nup62, RELA, PPP1CB, PPP1CA,
Protein Size: 1046

Molekulargewicht: 117 kDa

Gen-ID: 8473

NCBI Accession: NM_181672, NP_858058

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process