RFT1 Antikörper (N-Term)

Produktnummer ABIN2784093

Produktdetails anti-RFT1 Antikörper

Target: RFT1 (RFT1 Homolog (RFT1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Hund, Kaninchen, Ratte, Rind (Kuh), Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV

Homologie: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFT1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 541 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu RFT1

Target: RFT1 (RFT1 Homolog (RFT1))

Andere Bezeichnung: RFT1 (RFT1 Produkte)

Hintergrund: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a!!Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).[supplied by OMIM].
Alias Symbols: CDG1N
Protein Interaction Partner: PSMA3, UBC, NEDD4,
Protein Size: 541

Molekulargewicht: 60 kDa

Gen-ID: 91869

NCBI Accession: NM_052859, NP_443091

UniProt: Q96AA3