MED25 Antikörper (N-Term)

Produktnummer ABIN2784291

Produktdetails anti-MED25 Antikörper

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Meerschweinchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Sequenz: EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED25

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 747 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-MED25 Antikörper (ABIN2784291)

Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, (2013) (PubMed).

Details zu MED25

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Andere Bezeichnung: MED25 (MED25 Produkte)

Hintergrund: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Alias Symbols: ACID1, ARC92, CMT2B2, DKFZp434K0512, MGC70671, P78, PTOV2, TCBAP0758
Protein Interaction Partner: UBC, MED19, MED26, EPAS1, NOTCH1, DREB2A, CTDP1, MED6, THRA, RXRA, RARA, MED1, NR3C1, ESR1, CREBBP, SREBF1, MED25, MED15, MED16, MED13, MED12, MED7, MED17, MED23, MED14, CDK8, ZC3H13, QKI, TRIP4, TADA2A, MED28, OBFC1, TRRAP, UL48, MED9, MED8, HCFC1, MED10
Protein Size: 747

Molekulargewicht: 78 kDa

Gen-ID: 81857

NCBI Accession: NM_030973, NP_112235

UniProt: Q71SY5

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response