POLI Antikörper (N-Term)

Produktnummer ABIN2784869

Produktdetails anti-POLI Antikörper

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Kaninchen, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLI

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 740 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Andere Bezeichnung: POLI (POLI Produkte)

Synonyme: poli antikoerper, wu:fc27h11 antikoerper, zgc:110185 antikoerper, GB12093 antikoerper, NV16187 antikoerper, RAD30B antikoerper, RAD3OB antikoerper, Rad30b antikoerper, DNA polymerase iota antikoerper, polymerase (DNA directed) iota antikoerper, polymerase (DNA directed), iota antikoerper, POLI antikoerper, poli antikoerper, LOC551219 antikoerper, AFUA_4G11140 antikoerper, LOC5568276 antikoerper, Pol-iota antikoerper, Poli antikoerper

Hintergrund: POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Alias Symbols: RAD30B, RAD3OB
Protein Interaction Partner: TMEM239, TRIM39, ZBTB44, UBC, TRAF6, DAZAP2, RNF11, TRIM23, POLH, PCNA, APP, REV1, XRCC1, UBB, UBA52, CALCOCO2, DVL2,
Protein Size: 740

Molekulargewicht: 83 kDa

Gen-ID: 11201

NCBI Accession: NM_007195, NP_009126

UniProt: Q9UNA4

Pathways: DNA Reparatur