ADD2 Antikörper (Middle Region)

Produktnummer ABIN2784900

Produktdetails anti-ADD2 Antikörper

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Schwein, Hund, Meerschweinchen, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Sequenz: VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against ADD2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ADD2

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 726 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu ADD2

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Andere Bezeichnung: ADD2 (ADD2 Produkte)

Synonyme: si:dkey-202n14.5 antikoerper, ADDB antikoerper, 2900072M03Rik antikoerper, add97 antikoerper, beta-ADD antikoerper, adducin 2 antikoerper, adducin 2 (beta) antikoerper, ADD2 antikoerper, add2 antikoerper, Add2 antikoerper

Hintergrund: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
Alias Symbols: ADDB
Protein Interaction Partner: UBC, APP, RPH3A, PRKACA, PRKCD, PTPRZ1, FYN, CALM1, ADD2, ADD1,
Protein Size: 726

Molekulargewicht: 80 kDa

Gen-ID: 119

NCBI Accession: NM_001617, NP_001608

UniProt: P35612

Pathways: Regulation of Actin Filament Polymerization