Crossover junction endonuclease EME1 (EME1) (Middle Region) Antikörper

Produktnummer ABIN2785184

Produktdetails

Target: Crossover junction endonuclease EME1 (EME1)

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen, Rind (Kuh), Hund, Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: AYPSPQLLVQ AYQQCFSDKE RQNLLADIQV RRGEGVTSTS RRIGPELSRR

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against EME1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EME1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 570 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Antigendetails

Target: Crossover junction endonuclease EME1 (EME1)

Andere Bezeichnung: EME1 (EME1 Produkte)

Hintergrund: EME1 interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks.EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM].
Alias Symbols: FLJ31364, MMS4L, SLX2A
Protein Interaction Partner: vpr, MUS81, APP, SLX1A, EME1, SLX4, SUMO2, SLX1B,
Protein Size: 570

Molekulargewicht: 63 kDa

Gen-ID: 146956

NCBI Accession: NM_152463, NP_689676

UniProt: Q96AY2