NLRC4 Antikörper (C-Term)

Produktnummer ABIN2792097

Produktdetails anti-NLRC4 Antikörper

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NLRC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: QLNLAGNRVS SDGWLAFMGV FENLKQLVFF DFSTKEFLPD PALVRKLSQV

Spezifität: This antibody will recognize NLRC4 isoform 1 (116kD) and isoform 2 (40kD).

Homologie: Guinea Pig: 80%, Human: 100%, Mouse: 86%, Rat: 80%

Produktmerkmale: This is a rabbit polyclonal antibody against NLRC4. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NLRC4

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1024 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-NLRC4 Antikörper (ABIN2792097)

Hughes, Vivar, Kennis, Pratt-Thomas, Lowe, Shaner, Nietert, Spruill, Purves: "Inflammasomes are important mediators of cyclophosphamide-induced bladder inflammation." in: American journal of physiology. Renal physiology, Vol. 306, Issue 3, pp. F299-308, (2014) (PubMed).

Details zu NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Andere Bezeichnung: NLRC4 (NLRC4 Produkte)

Hintergrund: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.
Alias Symbols: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1
Protein Interaction Partner: UBC, XK, ALB, NLRC4, PSMC5, CASP8, NLRP4, PYCARD, NOD2, BCL10, CASP1, NLRP1, NLRP3, NOD1, NAIP,
Protein Size: 1024

Molekulargewicht: 116 kDa

Gen-ID: 58484

NCBI Accession: NM_021209, NP_067032

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome