Topoisomerase II alpha Antikörper (C-Term)

Produktnummer ABIN184179

Produktdetails anti-Topoisomerase II alpha Antikörper

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Schwein, Hund, Pferd, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: VSKAVTSKKS KGESDDFHMD FDSAVAPRAK SVRAKKPIKY LEESDEDDLF

Homologie: Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 87%, Pig: 100%, Rabbit: 100%, Rat: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against TOP2A. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TOP2A

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1531 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A (TOP2A Produkte)

Hintergrund: TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Alias Symbols: TOP2, TP2A
Protein Interaction Partner: UBC, SUMO2, SUMO3, EIF6, RPA3, RPA2, RPA1, SUMO1, LMNA, CTNNB1, ITGA4, FN1, CSNK2A1, VCAM1, SRPK1, RB1, ESR1, TOP2B, CSNK2A2, UPF3B, PRKCG, PRKCB, PRKCA, TERF2, BLM, NEDD8, COPS5, CUL5, FBXW7, ISG15, CUL2, VHL, TCEB1, GSK3B, SUMO4, SMARCAD1, CTCF, RING1,
Protein Size: 1531

Molekulargewicht: 174 kDa

Gen-ID: 7153

NCBI Accession: NM_001067, NP_001058

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases