DEK Antikörper (N-Term)

Produktnummer ABIN184277

Produktdetails anti-DEK Antikörper

Target: DEK (DEK Oncogene (DEK))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Rind (Kuh), Kaninchen, Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DEK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: AAEGEGTPTQ PASEKEPEMP GPREESEEEE DEDDEEEEEE EKEKSLIVEG

Kreuzreaktivität: Rind (Kuh), Human, Ratte (Rattus)

Homologie: Cow: 78%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Rabbit: 85%, Rat: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against DEK. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DEK

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-DEK Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 375 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-DEK Antikörper (ABIN184277)

Wang, Eckert, Zomorrodi, Xin, Pan, Shearer, Weisz, Maranus, Clawson: "Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways." in: PLoS ONE, Vol. 7, Issue 6, pp. e39446, (2012) (PubMed).

Details zu DEK

Target: DEK (DEK Oncogene (DEK))

Andere Bezeichnung: DEK (DEK Produkte)

Hintergrund: DEK was first identified in a fusion with the CAN nucleoporin protein in a specific subtype of acute myelogenous leukemia. DEK has also been shown to be an autoantigen in patients with pauciarticular onset juvenile rheumatoid arthritis. Further, the last 65 amino acids of DEK can partially reverse the mutation-prone phenotype of cells from patients with ataxia-telangiectasia
Alias Symbols: D6S231E
Protein Interaction Partner: CUL3, SPOP, UBC, EED, IRF2BP2, GANAB, PTPN12, CSNK2A2, CSNK2A1, TP53BP1, ESR1, VTN, S100A9, RPL37A, DHX15, FTSJ3, WIBG, TMED9, EXOSC2, UTP14A, THRAP3, CAND1, COPS5, CDK2, YWHAZ, Cebpb, ELAVL1, SUMO1, SUMO2, RAD21, HDGF, KAT2B, EP300, CREBBP, HIST1H4A, HDA
Protein Size: 375

Molekulargewicht: 43 kDa

Gen-ID: 7913

NCBI Accession: NM_003472, NP_003463

UniProt: P35659