NFX1 Antikörper (Middle Region)

Produktnummer ABIN2792551

Produktdetails anti-NFX1 Antikörper

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: TLTGVLEREM QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte (Rattus)

Homologie: Cow: 85%, Dog: 85%, Human: 100%, Mouse: 76%, Rabbit: 85%, Rat: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against NFX1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFX1

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1120 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Andere Bezeichnung: NFX1 (NFX1 Produkte)

Synonyme: NFX1 antikoerper, NFX2 antikoerper, 1300017N15Rik antikoerper, 3000003M19Rik antikoerper, NFX.1 antikoerper, TEG-42 antikoerper, Tex42 antikoerper, nuclear transcription factor, X-box binding 1 antikoerper, nuclear transcription factor, x-box binding 1 antikoerper, NFX1 antikoerper, CpipJ_CPIJ011986 antikoerper, nfx1 antikoerper, Nfx1 antikoerper

Hintergrund: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Alias Symbols: DKFZp779G2416, MGC20369, NFX2
Protein Interaction Partner: UBC, BMI1, HECW2, SRSF4, SRSF3, RPS19, RPS3A, RPL22, RPL17, RPL13, RPL7A, RPL6, RPL3, PABPC3, YBX1, NCL, HNRNPM, HSPA1A, HNRNPU, HNRNPH1, HNRNPA2B1, HIST1H2AE, EEF1A2, DMD, DDX5, FAM208B, PABPC1, RPL13A, LARP1, APC2, MATR3, HIST1H4E, USP9X, SIN3A, MAML3,
Protein Size: 1120

Molekulargewicht: 124 kDa

Gen-ID: 4799

NCBI Accession: NM_002504, NP_002495

UniProt: Q12986