SSRP1 Antikörper (Middle Region)

Produktnummer ABIN2792588

Produktdetails anti-SSRP1 Antikörper

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SSRP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: MKEYEGGRGE SSKRDKSKKK KKVKVKMEKK STPSRGSSSK SSSRQLSESF

Kreuzreaktivität: Rind (Kuh), Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus)

Homologie: Cow: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against SSRP1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SSRP1

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-SSRP1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Human Spleen.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 709 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu SSRP1

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Andere Bezeichnung: SSRP1 / FACT80 (SSRP1 Produkte)

Hintergrund: SSRP1 is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. SSRP1 contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. It also functions as a co-activator of the transcriptional activator p63. The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FACT, FACT80, T160
Protein Interaction Partner: SUMO2, SUMO3, ATRX, UBC, EED, RNF2, SEC24C, CUL1, CSDE1, ATP6V1B2, DIABLO, SBNO1, PBDC1, EXOSC10, CSNK2A1, PNKP, VCAM1, SRPK1, ITGA4, FMNL1, SUPT16H, CENPA, LEO1, PAF1, PHGDH, SF3B1, NCSTN, RTF1, RBM14, SAP18, CTR9, S100A9, NOP2, HMGB1, HIST1H2BB, DHX9, C
Protein Size: 709

Molekulargewicht: 81 kDa

Gen-ID: 6749

NCBI Accession: NM_003146, NP_003137

UniProt: Q08945

Pathways: Chromatin Binding