Tested Western blot Application Notes Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Sample (50 μg of whole cell lysate) A: Mouse brain 5 % SDS PAGE 107-10546 diluted at 1:1000 NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by Western blot analysis.A. 50 μg rat brain lysate/extract5 % SDS-PAGENCKAP1 antibody [C1C2], Internal (107-10546) dilution: 1:1000Sample (30 μg whole cell lysate)A:Hep G2 7.5 % SDS PAGE107-10546 diluted at 1:500
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.5 mg/mL
Buffer
0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.