Fluorescein Antikörper

Produktnummer ABIN2801850

Produktdetails

Target: Fluorescein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA

Spezifität: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Fluorescein conjugated Rabbit IgG and Fluorescein conjugated KLH.

Produktmerkmale: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Sterilität: Sterile filtered

Immunogen: Fluorescein conjugated Bovine Serum Albumin (BSA)
Immunogen Type: NativeProtein

Anwendungsinformationen

Applikationshinweise: This product has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] Donkey) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Konzentration: 90 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Antigendetails

Target: Fluorescein

Substanzklasse: Chemical

Hintergrund: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC