Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Spezifität
The monoclonal detects wild type and recombinant GFP. This antibody does not detect enhanced GFP.
Immunogen
GFP antibody was raised in mouse using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.
ELISA: 1:200,000, IHC: 1:1,000-1:5,000, WB: 1:3,000-1:30,000 Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
Lot specific
Buffer
0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.