SNAIL Antikörper

Produktnummer ABIN2854294

Produktdetails anti-SNAIL Antikörper

Target: SNAIL (SNAI1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SNAIL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to SNAI1 (snail family zinc finger 1)
SNAI1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: U87-MG , SK-N-SH , IMR32 , SK-N-AS , HeLa (starvation for 16 hr 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min) , SNAI1-transfected 293T

Validation: Comparison, Orthogonal, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.54 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-SNAIL Antikörper (ABIN2854294)

Chung, Lee, Hu, Chou, Lin, Shih: "NKX6.1 Represses Tumorigenesis, Metastasis, and Chemoresistance in Colorectal Cancer." in: International journal of molecular sciences, Vol. 21, Issue 14, (2020) (PubMed).

Roth, Jayaratna, Sundi, Cheng, Melquist, Choi, Porten, Nitti, Navai, Wszolek, Guo, Czerniak, McConkey, Dinney: "Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis." in: Oncotarget, Vol. 8, Issue 21, pp. 34205-34222, (2018) (PubMed).

Li, Yu, Huang, Su, Hsiao, Chang, Yu, Lin: "NKX6.1 functions as a metastatic suppressor through epigenetic regulation of the epithelial-mesenchymal transition." in: Oncogene, Vol. 35, Issue 17, pp. 2266-78, (2017) (PubMed).

Liu, Weng, Lin, Tang, Chen, Liang, Ku, Lin: "Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells." in: Scientific reports, Vol. 7, pp. 41437, (2017) (PubMed).

Details zu SNAIL

Target: SNAIL (SNAI1)

Andere Bezeichnung: snail family transcriptional repressor 1 (SNAI1 Produkte)

Hintergrund: The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.

Cellular Localization: Nucleus

Molekulargewicht: 29 kDa

Gen-ID: 6615

UniProt: O95863

Pathways: Negative Regulation of intrinsic apoptotic Signaling