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beta Actin Antikörper (N-Term)

Name: beta Actin Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN2854709
Price: 659.23 EUR
Availability: InStock

KO Validated ACTB Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody antibody diluted at 1:1000 (beta Actin Antikörper (N-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin, antibody (Green) at 1:1000 dilution. (beta Actin Antikörper (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin, antibody at 1:500 dilution. (beta Actin Antikörper (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (beta Actin Antikörper (N-Term))

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody , diluted at 1:1000. (beta Actin Antikörper (N-Term))

IHC-P Image beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. (beta Actin Antikörper (N-Term))

IHC

6 Abbildungen

(4 Referenzen)

Produktnummer ABIN2854709

Datenblatt (PDF)

Target Alle beta Actin (ACTB) Antikörper anzeigen

beta Actin (ACTB) (Actin, beta (ACTB))
Bindungsspezifität
38

16

15

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reaktivität
176

127

108

56

43

42

37

25

24

22

18

17

12

10

9

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human
Wirt
112

79

5

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
110

86
Polyklonal
Konjugat
124

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser beta Actin Antikörper ist unkonjugiert
Applikation
164

70

51

45

34

30

26

22

13

13

8

6

6

5

5

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität

Cat, Hund, Drosophila melanogaster, Human, Maus

Produktmerkmale

Rabbit Polyclonal antibody to beta Actin (actin, beta)
beta Actin antibody

Aufreinigung

Purified by antigen-affinity chromatography.

Güteklasse

KO Validated

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

Isotyp

IgG

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anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
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anti-Actin, beta (ACTB) antibody
ACTB Reaktivität: Human, Maus, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch WB, ELISA Wirt: Maus Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reaktivität: Human WB, ELISA, IHC, IF, IP, FACS Wirt: Maus Monoclonal 1D12E12B5 unconjugated

Applikationshinweise

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: NIH-3T3 , Raji , 293T
Validation: KO/KD

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.16 mg/mL

Buffer

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Yu, Luan, Liu, Su, Li: "MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal." in: Pharmacology, Vol. 105, Issue 3-4, pp. 190-201, (2020) (PubMed).

Tao, Cheng, Yang: "Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway." in: Clinical interventions in aging, Vol. 15, pp. 373-385, (2020) (PubMed).

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung

actin beta (ACTB Produkte)

Hintergrund

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Cellular Localization: Cytoplasm , cytoskeleton

Molekulargewicht

42 kDa

Gen-ID

60

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction