RAD51 Antikörper (Center)

Produktnummer ABIN2854762

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RAD51 in WB, IP, IHC (p), IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für RAD51 Antikörper (Center) (ABIN2854762)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Huhn, Hund, Kaninchen, Ratte (Rattus), Zebrafisch (Danio rerio), Rind (Kuh), Xenopus tropicalis

Kreuzreaktivität (Details): Chicken (95 %), Dog (98 %), Rabbit (97 %), Rat (98 %), Zebrafish (90 %), Bovine (98 %), Xenopus tropicalis (97 %)

Produktmerkmale: Rabbit Polyclonal antibody to Rad51 (RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae))
Rad51 antibody [N1C2]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.57 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-RAD51 Antikörper (ABIN2854762)

Chen, Lin, Liang, Villalobos, Villaflores, Lou, Lai, Hsiao: "UVB Irradiation Induced Cell Damage and Early Onset of Junbb Expression in Zebrafish." in: Animals : an open access journal from MDPI, Vol. 10, Issue 6, (2020) (PubMed).

Nacson, Krais, Bernhardy, Clausen, Feng, Wang, Nicolas, Cai, Tricarico, Hua, DiMarcantonio, Martinez, Zong, Handorf, Bellacosa, Testa, Nussenzweig, Gupta, Sykes, Johnson: "BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance." in: Cell reports, Vol. 24, Issue 13, pp. 3513-3527.e7, (2019) (PubMed).

Chakraborty, Tapryal, Venkova, Horikoshi, Pandita, Sarker, Sarkar, Pandita, Hazra: "Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes." in: Nature communications, Vol. 7, pp. 13049, (2018) (PubMed).

Ge, Che, Du: "The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144957, (2015) (PubMed).

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: Rad51

Hintergrund: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

Molekulargewicht: 37 kDa

Gen-ID: 5888

Pathways: DNA Reparatur