SEC13 Antikörper (Center)

Produktnummer ABIN2854912

Produktdetails anti-SEC13 Antikörper

Target: SEC13 (SEC13 Homolog (SEC13))

Bindungsspezifität: Center

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SEC13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität: Maus, Ratte (Rattus), Xenopus laevis, Zebrafisch (Danio rerio), Rind (Kuh), Xenopus tropicalis

Kreuzreaktivität (Details): Mouse (97 %), Rat (97 %), Xenopus laevis (88 %), Zebrafish (87 %), Bovine (96 %), Xenopus tropicalis (89 %)

Produktmerkmale: Rabbit Polyclonal antibody to SEC13L1 (SEC13 homolog (S. cerevisiae))
SEC13L1 antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SEC13L1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-SEC13 Antikörper (ABIN2854912)

Wolfson, Chantranupong, Wyant, Gu, Orozco, Shen, Condon, Petri, Kedir, Scaria, Abu-Remaileh, Frankel, Sabatini: "KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1." in: Nature, Vol. 543, Issue 7645, pp. 438-442, (2017) (PubMed).

Details zu SEC13

Target: SEC13 (SEC13 Homolog (SEC13))

Andere Bezeichnung: SEC13L1 (SEC13 Produkte)

Synonyme: D3S1231E antikoerper, SEC13L1 antikoerper, SEC13R antikoerper, npp-20 antikoerper, 1110003H02Rik antikoerper, Sec13l1 antikoerper, Sec13r antikoerper, Protein SEC13 homolog antikoerper, SEC13 homolog, nuclear pore and COPII coat complex component antikoerper, npp-20 antikoerper, SEC13 antikoerper, Sec13 antikoerper

Hintergrund: The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.

Molekulargewicht: 36 kDa

Gen-ID: 6396