VCP Antikörper (C-Term)

Produktnummer ABIN2854929

Produktdetails anti-VCP Antikörper

Target: VCP (Valosin Containing Protein (VCP))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VCP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

Spezifität: Knockdown/Knockout validation was supported by customer review data.

Kreuzreaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to VCP (valosin containing protein)
VCP antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Mouse brain , Rat brain

Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-VCP Antikörper (ABIN2854929)

Chen, Morales, Guerrero, Sun, McCarty: "PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells." in: Cancer research, Vol. 78, Issue 14, pp. 3809-3822, (2019) (PubMed).

Details zu VCP

Target: VCP (Valosin Containing Protein (VCP))

Andere Bezeichnung: valosin containing protein (VCP Produkte)

Hintergrund: The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Cellular Localization: Cytoplasm , cytosol , Nucleus

Molekulargewicht: 89 kDa

Gen-ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway