Lamin A/C Antikörper

Produktnummer ABIN2854943

Produktdetails anti-Lamin A/C Antikörper

Target: Lamin A/C (LMNA)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Lamin A + C
Lamin A + C antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: NIH-3T3

Validation: Comparison, KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.73 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-Lamin A/C Antikörper (ABIN2854943)

Zhang, Li, Guan, Zhou, Wang, Yang, Zhen, Dai, Zhao, Jiang, Xu: "MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation." in: Frontiers in physiology, Vol. 11, pp. 510, (2020) (PubMed).

Kuo, Ling, Chiang, Chung, Lee, Chu, Wu, Chen, Lai, Tsai, Cheng, Lin: "Metastatic Colorectal Cancer Rewrites Metabolic Program Through a Glut3-YAP-dependent Signaling Circuit." in: Theranostics, Vol. 9, Issue 9, pp. 2526-2540, (2020) (PubMed).

Bueno, Martínez-Morga, Martínez: "Non-proliferative neurogenesis in human periodontal ligament stem cells." in: Scientific reports, Vol. 9, Issue 1, pp. 18038, (2019) (PubMed).

Wu, Yen, Kou, Wu: "Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130599, (2016) (PubMed).

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: lamin A/C (LMNA Produkte)

Hintergrund: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Cellular Localization: Nucleus

Molekulargewicht: 74 kDa

Gen-ID: 4000

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus