E2F1 Antikörper

Produktnummer ABIN2854981

Produktdetails anti-E2F1 Antikörper

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Kreuzreaktivität: Human

Produktmerkmale: Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)
E2F1 antibody [N1N3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: E2F1-transfected 293T

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F transcription factor 1 (E2F1 Produkte)

Synonyme: CG6376 antikoerper, DRTF1/E2F antikoerper, DmE2F-1 antikoerper, Dmel\\CG6376 antikoerper, Dp antikoerper, E(Sev-CycE)3A antikoerper, E(var)3-93E antikoerper, E(var)3-95E antikoerper, E(var)93E antikoerper, E2F antikoerper, E2F-1 antikoerper, E2F1 antikoerper, E2f1 antikoerper, Evar(3)164 antikoerper, dE2F antikoerper, dE2F1 antikoerper, dE2f antikoerper, dE2f1 antikoerper, de2f1 antikoerper, def21 antikoerper, drosE2F1 antikoerper, e2f antikoerper, e2f1 antikoerper, l(3)07172 antikoerper, l(3)j3B1 antikoerper, l(3)j3C2 antikoerper, l(3)rM729 antikoerper, RBAP1 antikoerper, RBBP3 antikoerper, RBP3 antikoerper, mKIAA4009 antikoerper, xE2F antikoerper, ATE2FB antikoerper, E2F TRANSCRIPTION FACTOR-1 E2F1 antikoerper, E2F transcription factor 1 antikoerper, E2FB antikoerper, E2FB TRANSCRIPTION FACTOR antikoerper, E2F transcription factor 1 antikoerper, E2F transcription factor 1 L homeolog antikoerper, E2f1 antikoerper, E2F1 antikoerper, e2f1.L antikoerper

Hintergrund: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Cellular Localization: Nucleus

Molekulargewicht: 47 kDa

Gen-ID: 1869

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie