OCT4 Antikörper

Produktnummer ABIN2855068

Produktdetails anti-OCT4 Antikörper

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)
Oct4 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: mouse ESC , human ESC , NT2D1

Validation: KO/KD, Orthogonal, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.49 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-OCT4 Antikörper (ABIN2855068)

Feng, Su, Lin, Lin, Wu, Tung, Li, Shieh, Shiau: "Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer." in: BMC cancer, Vol. 19, Issue 1, pp. 791, (2020) (PubMed).

Lee, Liu, Chang, Nieh, Lin, Jao, Chen, Liu: "Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative." in: Oncotarget, Vol. 7, Issue 17, pp. 23512-20, (2018) (PubMed).

Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

Chen, Huang, Lin: "Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma." in: International journal of oral science, Vol. 6, Issue 4, pp. 212-8, (2015) (PubMed).

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: POU class 5 homeobox 1 (POU5F1 Produkte)

Synonyme: OCT3 antikoerper, OCT4 antikoerper, OTF-3 antikoerper, OTF3 antikoerper, OTF4 antikoerper, Oct-3 antikoerper, Oct-4 antikoerper, POU5F1 antikoerper, gp9 antikoerper, gp-9 antikoerper, oct4 antikoerper, pou2 antikoerper, POU-2 antikoerper, cb197 antikoerper, spg/pou2 antikoerper, chunp6868 antikoerper, wu:fd18d06 antikoerper, NF-A3 antikoerper, Oct-3/4 antikoerper, Oct3 antikoerper, Oct3/4 antikoerper, Oct4 antikoerper, Otf-3 antikoerper, Otf-4 antikoerper, Otf3 antikoerper, Otf3-rs7 antikoerper, Otf3g antikoerper, Otf4 antikoerper, POU class 5 homeobox 1 antikoerper, POU domain, class 5, transcription factor 3 antikoerper, POU domain, class 5, transcription factor 1 antikoerper, POU5F1 antikoerper, pou5f3 antikoerper, Pou5f1 antikoerper

Hintergrund: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Cellular Localization: Nucleus

Molekulargewicht: 39 kDa

Gen-ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance