MTOR Antikörper (C-Term)

Produktnummer ABIN2855088

Produktdetails anti-MTOR Antikörper

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MTOR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Produktmerkmale: Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-MTOR Antikörper (ABIN2855088)

Wang, Cao, Shu, Zhu, Peng, Ran, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes." in: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1399-1412, (2020) (PubMed).

Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

Peng, Chen, Wang, Zhu, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes." in: Aging, Vol. 11, Issue 23, pp. 11520-11540, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

Details zu MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Andere Bezeichnung: mTOR (mTOR Produkte)

Synonyme: FRAP1 antikoerper, FRAP antikoerper, FRAP2 antikoerper, RAFT1 antikoerper, RAPT1 antikoerper, 2610315D21Rik antikoerper, AI327068 antikoerper, Frap1 antikoerper, flat antikoerper, frap1 antikoerper, tor antikoerper, wu:fc22h08 antikoerper, mechanistic target of rapamycin kinase antikoerper, mechanistic target of rapamycin antikoerper, mechanistic target of rapamycin (serine/threonine kinase) antikoerper, MTOR antikoerper, Mtor antikoerper, mtor antikoerper

Hintergrund: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side

Molekulargewicht: 289 kDa

Gen-ID: 2475

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effekt