Fibrillarin Antikörper

Produktnummer ABIN2855179

Produktdetails anti-Fibrillarin Antikörper

Target: Fibrillarin (FBL)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: IP/MS validation was supported by references (PMID:30377378)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Fibrillarin (fibrillarin)
Fibrillarin antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Rat2 , 293T , H1299 , NIH-3T3

Validation: IP/MS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: fibrillarin (FBL Produkte)

Hintergrund: This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.

Cellular Localization: Nucleus , nucleolus

Molekulargewicht: 34 kDa

Gen-ID: 2091

UniProt: P22087

Pathways: Ribonucleoside Biosynthetic Process