ARHGEF18 Antikörper (Center)

Produktnummer ABIN2855329

Produktdetails anti-ARHGEF18 Antikörper

Target: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Bindungsspezifität: Center

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARHGEF18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktmerkmale: Rabbit Polyclonal antibody to ARHGEF18 (Rho/Rac guanine nucleotide exchange factor (GEF) 18)
ARHGEF18 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ARHGEF18. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.43 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ARHGEF18 Antikörper (ABIN2855329)

Haas, Zihni, Ruppel, Hartmann, Ebnet, Tada, Balda, Matter: "Interplay between Extracellular Matrix Stiffness and JAM-A Regulates Mechanical Load on ZO-1 and Tight Junction Assembly." in: Cell reports, Vol. 32, Issue 3, pp. 107924, (2020) (PubMed).

Lu, Wawro, Morgens, Portela, Bassik, Pfeffer: "Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs." in: eLife, Vol. 7, (2018) (PubMed).

Zihni, Vlassaks, Terry, Carlton, Leung, Olson, Pichaud, Balda, Matter: "An apical MRCK-driven morphogenetic pathway controls epithelial polarity." in: Nature cell biology, Vol. 19, Issue 9, pp. 1049-1060, (2017) (PubMed).

Details zu ARHGEF18

Target: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Andere Bezeichnung: ARHGEF18 (ARHGEF18 Produkte)

Hintergrund: Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified.

Cellular Localization: Cytoplasm

Molekulargewicht: 131 kDa

Gen-ID: 23370

Pathways: Neurotrophin Signalübertragung