Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (C-Term) Antikörper

Produktnummer ABIN2855378

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (C-Term) Antikörper (ABIN2855378)

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Bindungsspezifität: C-Term

Kreuzreaktivität: Rind (Kuh)

Kreuzreaktivität (Details): Bovine (95 %)

Produktmerkmale: Rabbit polyclonal antibody to CBL-B (Cas-Br-M (murine) ecotropic retroviral transforming sequence b)
Cbl-b antibody [C2C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Cbl-b. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Kommentare:

Positive Control: HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.72 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Andere Bezeichnung: CBL-B

Hintergrund: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

Cellular Localization: Cytoplasm

Molekulargewicht: 109 kDa

Gen-ID: 868