LIG3 Antikörper (C-Term)

Produktnummer ABIN2855575

Produktdetails anti-LIG3 Antikörper

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Kreuzreaktivität: Hamster, Human

Produktmerkmale: Rabbit Polyclonal antibody to DNA ligase III (ligase III, DNA, ATP-dependent)
DNA ligase III antibody [C2C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase III. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T

Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.15 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-LIG3 Antikörper (ABIN2855575)

Abdou, Poirier, Hendzel, Weinfeld: "DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair." in: Nucleic acids research, Vol. 43, Issue 2, pp. 875-92, (2015) (PubMed).

Details zu LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Andere Bezeichnung: DNA ligase 3 (LIG3 Produkte)

Synonyme: orf36 antikoerper, DDBDRAFT_0218523 antikoerper, DDBDRAFT_0232266 antikoerper, DDB_0218523 antikoerper, DDB_0232266 antikoerper, LIG3 antikoerper, wu:fb12c02 antikoerper, wu:fd10b03 antikoerper, wu:fm79b09 antikoerper, LIG2 antikoerper, D11Wsu78e antikoerper, An02g06890 antikoerper, AO090003000668 antikoerper, lig3 antikoerper, DNA ligase III antikoerper, BRCT domain-containing protein antikoerper, DNA ligase 3 antikoerper, ligase III, DNA, ATP-dependent antikoerper, ligase III, DNA, ATP-dependent L homeolog antikoerper, orf36 antikoerper, lig3 antikoerper, LIG3 antikoerper, Lig3 antikoerper, Bm1_22840 antikoerper, ANI_1_2644024 antikoerper, AOR_1_1174154 antikoerper, LOC100634887 antikoerper, lig3.L antikoerper

Hintergrund: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Nucleus

Molekulargewicht: 113 kDa

Gen-ID: 3980

UniProt: P49916

Pathways: DNA Reparatur