Calpain 5 Antikörper

Produktnummer ABIN2855597

Der Kaninchen Polyklonal Anti-Calpain 5-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, Calpain 5 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Calpain 5 Antikörper (ABIN2855597)

Target: Calpain 5 (CAPN5)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calpain 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Calpain 5 Antikörper

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit polyclonal antibody to Calpain-5 (calpain 5)
Calpain-5 antibody [N1C1]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: mouse uterus , RMS-13

Validation: Orthogonal, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.39 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-Calpain 5 Antikörper (ABIN2855597)

Velez, Bassuk, Schaefer, Brooks, Gakhar, Mahajan, Kahn, Tsang, Ferguson, Mahajan: "A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay." in: Cold Spring Harbor molecular case studies, Vol. 4, Issue 3, (2019) (PubMed).

Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, (2016) (PubMed).

Details zu Calpain 5

Target: Calpain 5 (CAPN5)

Andere Bezeichnung: calpain 5

Hintergrund: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

Molekulargewicht: 73 kDa

Gen-ID: 726

UniProt: O15484