c-MYC Antikörper (Center)

Produktnummer ABIN2855631

Produktdetails anti-c-MYC Antikörper

Highlights:

• High quality c-MYC Primärantikörper for the detection of MYC.
• Reliable product with high standard validation data

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Bindungsspezifität: Center

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser c-MYC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Kreuzreaktivität: Schimpanse

Kreuzreaktivität (Details): Chimpanzee (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to c-Myc (v-myc myelocytomatosis viral oncogene homolog (avian))
c-Myc antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.

Isotyp: IgG

Produktspezifische Information:

What can the c-MYC Antibody ABIN2855631 be used for?: This polyclonal c-MYC Antibody detects c-MYC. The c-MYC Antibody has been validated for various applications and can be used for the detection of c-MYC and derivatives by Western Blotting, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Chromatin Immunoprecipitation.

What validation data is available for this c-MYC Antibody?: The Primärantikörper is referenced in 5 publications and is characterized by a proven, very high reliability. It has currently 8 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. c-MYC Antibody for the detection of c-MYC and derivatives.

What is the function of c-MYC?: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008].

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , NIH-3T3 , Raw264.7 , HeLa , HeLa nuclear extract

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-c-MYC Antikörper (ABIN2855631)

Chen, Ding, Chen, Hu, Lu, Wu, Zhang, Yang, Wu, Peng, Xie, Zhou, Wu, Zheng: "ACSL4 promotes hepatocellular carcinoma progression via c-Myc stability mediated by ERK/FBW7/c-Myc axis." in: Oncogenesis, Vol. 9, Issue 4, pp. 42, (2020) (PubMed).

Seira, Yamagata, Fukushima, Araki, Kurata, Yanagisawa, Mashimo, Nakamura, Regan, Murayama, Fujino: "Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region." in: Pharmacology research & perspectives, Vol. 6, Issue 6, pp. e00441, (2019) (PubMed).

Ma, Wang, Chen, Li, Hur, Saijilafu: "Differential Roles of Glycogen Synthase Kinase 3 Subtypes Alpha and Beta in Cortical Development." in: Frontiers in molecular neuroscience, Vol. 10, pp. 391, (2017) (PubMed).

Chung, Liu, Lin, Chi, Huang, Yang, Yeh, Tan, Lin: "ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance." in: Oncotarget, Vol. 7, Issue 16, pp. 22448-59, (2017) (PubMed).

Wu, Lee, Hua, Kuo, Lin: "Macrophage Infiltration Induces Gastric Cancer Invasiveness by Activating the β-Catenin Pathway." in: PLoS ONE, Vol. 10, Issue 7, pp. e0134122, (2016) (PubMed).

Details zu c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Andere Bezeichnung: C-Myc (MYC Produkte)

Hintergrund: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Cellular Localization: Nucleus

Molekulargewicht: 49 kDa

Gen-ID: 4609

Pathways: p53 Signalweg, Zellzyklus, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effekt