ETS2 Antikörper (Center)

Produktnummer ABIN2855845

Produktdetails anti-ETS2 Antikörper

Target: ETS2 (V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 (ETS2))

Bindungsspezifität: Center

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ETS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Produktmerkmale: Rabbit Polyclonal antibody to ETS2 (v-ets erythroblastosis virus E26 oncogene homolog 2 (avian))
ETS2 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: HeLa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ETS2 Antikörper (ABIN2855845)

Gluck, Glathar, Tsompana, Nowak, Garrett-Sinha, Buck, Sinha: "Molecular dissection of the oncogenic role of ETS1 in the mesenchymal subtypes of head and neck squamous cell carcinoma." in: PLoS genetics, Vol. 15, Issue 7, pp. e1008250, (2019) (PubMed).

Yuan, Jiang, Mayakonda, Huang, Ding, Lin, Yu, Lu, Loh, Chow, Savage, Tyner, Lin, Koeffler: "Super-Enhancers Promote Transcriptional Dysregulation in Nasopharyngeal Carcinoma." in: Cancer research, Vol. 77, Issue 23, pp. 6614-6626, (2018) (PubMed).

Helbig, Wockner, Bouendeu, Hille-Betz, McCue, French, Edwards, Pickett, Reddel, Chenevix-Trench, Dörk, Beesley: "Functional dissection of breast cancer risk-associated TERT promoter variants." in: Oncotarget, Vol. 8, Issue 40, pp. 67203-67217, (2017) (PubMed).

Details zu ETS2

Target: ETS2 (V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 (ETS2))

Andere Bezeichnung: ETS2 (ETS2 Produkte)

Hintergrund: ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Cellular Localization: Nucleus

Molekulargewicht: 53 kDa

Gen-ID: 2114

Pathways: EGFR Signaling Pathway, Myometrial Relaxation and Contraction