GLUD1 Antikörper

Produktnummer ABIN2856181

Produktdetails anti-GLUD1 Antikörper

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Drosophila melanogaster, Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Glutamate Dehydrogenase (glutamate dehydrogenase 1)
Glutamate Dehydrogenase antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.07 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-GLUD1 Antikörper (ABIN2856181)

Geiszler, Ugun-Klusek, Lawler, Pardon, Yuchun, Bai, Daykin, Auer, Bedford: "Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons." in: Scientific reports, Vol. 8, Issue 1, pp. 4833, (2019) (PubMed).

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: glutamate dehydrogenase 1 (GLUD1 Produkte)

Hintergrund: L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]

Cellular Localization: Mitochondrion matrix

Molekulargewicht: 61 kDa

Gen-ID: 2746

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt