PDK1 Antikörper

Produktnummer ABIN2856238

Produktdetails anti-PDK1 Antikörper

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Based on sequence homology, this antibody may cross react with other PDK family members.

Kreuzreaktivität: Human

Produktmerkmale: Rabbit polyclonal antibody to PDK1 (pyruvate dehydrogenase kinase, isozyme 1)
PDK1 antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate dehydrogenase kinase 1 / PDK1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HeLaS3 , Molt-4 , human PDK1 transfected 293T cells

Validation: Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Andere Bezeichnung: pyruvate dehydrogenase kinase 1 (PDK1 Produkte)

Hintergrund: Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.

Cellular Localization: Mitochondrion matrix

Molekulargewicht: 49 kDa

Gen-ID: 5163

UniProt: Q15118

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effekt