NAPA Antikörper (Center)

Produktnummer ABIN2856408

Produktdetails anti-NAPA Antikörper

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Bindungsspezifität: Center

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Rhesusaffen, Schimpanse

Kreuzreaktivität (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)
alpha SNAP antibody [N2C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:1000-1:10000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Andere Bezeichnung: alpha SNAP (NAPA Produkte)

Synonyme: SNAPA antikoerper, wu:fa26c04 antikoerper, zgc:63906 antikoerper, napb antikoerper, 1500039N14Rik antikoerper, AW209189 antikoerper, RA81 antikoerper, SNARE antikoerper, a-SNAP antikoerper, hyh antikoerper, alpha-SNAP antikoerper, Alpha-SNAP antikoerper, NSF attachment protein alpha antikoerper, N-ethylmaleimide-sensitive factor attachment protein, alpha b antikoerper, NSF attachment protein alpha L homeolog antikoerper, N-ethylmaleimide-sensitive factor attachment protein, alpha antikoerper, N-ethylmaleimide sensitive fusion protein attachment protein alpha antikoerper, soluble NSF attachment protein antikoerper, NAPA antikoerper, napab antikoerper, napa.L antikoerper, napa antikoerper, EHI_000300 antikoerper, Napa antikoerper, LOC102577508 antikoerper

Hintergrund: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different.

Cellular Localization: Membrane, Peripheral membrane protein

Molekulargewicht: 33 kDa

Gen-ID: 8775

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization