VPS35 Antikörper (C-Term)

Produktnummer ABIN2856562

Produktdetails anti-VPS35 Antikörper

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VPS35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Zebrafisch (Danio rerio), Huhn, Rind (Kuh)

Kreuzreaktivität (Details): Zebrafish (86 %), Chicken (93 %), Bovine (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))
VPS35 antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: A549 , H1299 , HCT116 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-VPS35 Antikörper (ABIN2856562)

Lenoir, Ustunel, Rajesh, Kaur, Moreau, Gruenberg, Overduin: "Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting." in: Nature communications, Vol. 9, Issue 1, pp. 993, (2018) (PubMed).

Details zu VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Andere Bezeichnung: VPS35 (VPS35 Produkte)

Hintergrund: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein

Molekulargewicht: 92 kDa

Gen-ID: 55737