VPS35 Antikörper (C-Term)

Produktnummer ABIN2856562

Produktdetails anti-VPS35 Antikörper

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VPS35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Zebrafisch (Danio rerio), Huhn, Rind (Kuh)

Kreuzreaktivität (Details): Zebrafish (86 %), Chicken (93 %), Bovine (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))
VPS35 antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: A549 , H1299 , HCT116 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-VPS35 Antikörper (ABIN2856562)

Lenoir, Ustunel, Rajesh, Kaur, Moreau, Gruenberg, Overduin: "Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting." in: Nature communications, Vol. 9, Issue 1, pp. 993, (2018) (PubMed).

Details zu VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Andere Bezeichnung: VPS35 (VPS35 Produkte)

Synonyme: MEM3 antikoerper, PARK17 antikoerper, vps35 antikoerper, MGC81959 antikoerper, bZ34G2.3 antikoerper, si:rp71-34g2.3 antikoerper, wu:fa66e03 antikoerper, wu:fb95c08 antikoerper, zgc:136268 antikoerper, 23.m05999 antikoerper, Mem3 antikoerper, VPS35 antikoerper, DKFZp459E1510 antikoerper, CG5625 antikoerper, DVps35 antikoerper, Dmel\\CG5625 antikoerper, Dvps35 antikoerper, dvps35 antikoerper, AI647796 antikoerper, mem3 antikoerper, VPS35, retromer complex component antikoerper, VPS35 retromer complex component L homeolog antikoerper, VPS35 retromer complex component antikoerper, vacuolar protein sorting 35 homolog (S. cerevisiae) antikoerper, vacuolar protein sorting-associated protein 35 antikoerper, vacuolar protein sorting 35 antikoerper, Vacuolar protein sorting 35 antikoerper, hypothetical protein antikoerper, VPS35 retromer complex component S homeolog antikoerper, retromer complex subunit Vps35 antikoerper, VPS35 antikoerper, vps35.L antikoerper, vps35 antikoerper, Tc00.1047053511367.170 antikoerper, Tb10.70.5460 antikoerper, BBOV_IV009740 antikoerper, CC1G_12185 antikoerper, PTRG_08682 antikoerper, CMU_017400 antikoerper, PHATR_43830 antikoerper, MCYG_00751 antikoerper, MGYG_01212 antikoerper, EHI_086580 antikoerper, EHI_002990 antikoerper, EHI_041950 antikoerper, EHI_096520 antikoerper, Bm1_44045 antikoerper, LOC100285267 antikoerper, Vps35 antikoerper, PGTG_10018 antikoerper, Tsp_09825 antikoerper, vps35.S antikoerper

Hintergrund: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein

Molekulargewicht: 92 kDa

Gen-ID: 55737