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RPA1 Antikörper (C-Term)

Name: RPA1 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN2856633
Price: 659.23 EUR
Availability: InStock

RPA1 Reaktivität: Human WB, IF, IP, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: mouse liver 7. (RPA1 Antikörper (C-Term))

IP Image Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3], Western blot analysis was performed using RPA70 antibody [C1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent. (RPA1 Antikörper (C-Term))

IHC-P Image RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis. (RPA1 Antikörper (C-Term))

IHC

IHC-P Image RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis. (RPA1 Antikörper (C-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit , antibody at 1:100 dilution. (RPA1 Antikörper (C-Term))

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit , antibody at 1:200 dilution. (RPA1 Antikörper (C-Term))

WB Image RPA70 antibody detects RPA70 protein by western blot analysis. (RPA1 Antikörper (C-Term))

7 Abbildungen

Produktnummer ABIN2856633

Datenblatt (PDF)

Target Alle RPA1 Antikörper anzeigen

RPA1 (Replication Protein A1, 70kDa (RPA1))
Bindungsspezifität
16

9

6

4

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reaktivität
75

24

14

4

3

3

2

2

2

2

2

2

2

2

1
Human
Wirt
62

12

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
61

15
Polyklonal
Konjugat
45

4

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser RPA1 Antikörper ist unkonjugiert
Applikation
63

27

20

14

14

13

13

12

9

7

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität

Human, Maus

Produktmerkmale

Rabbit Polyclonal antibody to RPA70 (replication protein A1, 70 kDa)
RPA70 antibody [C1C3]

Aufreinigung

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

Isotyp

IgG

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Applikationshinweise

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: Mouse liver , 293T , A431 , HeLa , HepG2

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.4 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

RPA1 (Replication Protein A1, 70kDa (RPA1))

Andere Bezeichnung

replication protein A1 (RPA1 Produkte)

Hintergrund

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair, it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Cellular Localization: Nucleus

Molekulargewicht

68 kDa

Gen-ID

6117

UniProt

P27694

Pathways

Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA