Pyruvate Kinase Antikörper (Center)

Produktnummer ABIN2856815

Der Kaninchen Polyklonal Anti-Pyruvate Kinase-Antikörper wurde für WB, IF, IHC (p) und ICC validiert. Er ist geeignet, Pyruvate Kinase in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Pyruvate Kinase Antikörper (Center) (ABIN2856815)

Target: Pyruvate Kinase (PK)

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Pyruvate Kinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-Pyruvate Kinase Antikörper

Bindungsspezifität: Center

Spezifität: This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Produktmerkmale: Rabbit Polyclonal antibody to Pyruvate Kinase (liver/RBC) (pyruvate kinase, liver and RBC)
Pyruvate Kinase (liver/RBC) antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , rat liver

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Andere Bezeichnung: Pyruvate Kinase

Hintergrund: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 62 kDa

Gen-ID: 5313