GAPDH Antikörper

Produktnummer ABIN2857072

Produktdetails anti-GAPDH Antikörper

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Kreuzreaktivität: Bakterien, C. elegans, Chanos chanos, Huhn, Rind (Kuh), Hund, Drosophila melanogaster, E. coli, Fisch, Hamster, Human, Insekten, Affe, Moskito, Maus, Nematode, Ochotona princeps, Schwein, Pflanzen, Kaninchen, Ratte, Saccharomyces cerevisiae, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
GAPDH antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.11 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-GAPDH Antikörper (ABIN2857072)

Zhang, Dai, Zhao, Wang, Dou, Zhuang, Chen, Zhao et al.: "MiR-874-3p represses the migration and invasion yet promotes the apoptosis and cisplatin sensitivity via being sponged by long intergenic non-coding RNA 00922 (LINC00922) and targeting ..." in: Bioengineered, Vol. 13, Issue 3, pp. 7082-7104, (2022) (PubMed).

Nakamura, Sato, Kobayashi, Kaneko, Ito, Soma, Okada, Miyamoto, Oya, Matsumoto, Nakamura, Kanaji, Miyamoto: "Vitamin D protects against immobilization-induced muscle atrophy via neural crest-derived cells in mice." in: Scientific reports, Vol. 10, Issue 1, pp. 12242, (2020) (PubMed).

Liao, Zhou, Zhang: "Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2." in: Molecular medicine reports, Vol. 22, Issue 2, pp. 803-809, (2020) (PubMed).

Xu, Li, Wang, Wu, Wang, Chen, Deng, Zhang, Wu, Wan, Liu, Huang, Hu, Zeng, Zhou: "Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock‑in mouse model." in: International journal of molecular medicine, Vol. 46, Issue 3, pp. 1118-1134, (2020) (PubMed).

Liang, Chang, Sheu, Lin, Chung, Teng, Suk: "The Antihistamine Deptropine Induces Hepatoma Cell Death through Blocking Autophagosome-Lysosome Fusion." in: Cancers, Vol. 12, Issue 6, (2020) (PubMed).

Liu, Du, Rao, Liu, Qu: "Long non-coding RNA NPBWR1-2 affects the development of ovarian cancer via multiple microRNAs." in: Oncology letters, Vol. 20, Issue 1, pp. 685-692, (2020) (PubMed).

Chang, Kam, Chao, Zhao, Chen, Chung, Li, Wu, Lee: "Berberine Derivatives Suppress Cellular Proliferation and Tumorigenesis In Vitro in Human Non-Small-Cell Lung Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 12, (2020) (PubMed).

Low, Lee, Lee, Wang, Chen, Chien, Li, Chang: "Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells." in: Cancers, Vol. 12, Issue 6, (2020) (PubMed).

Möltgen, Piumatti, Massafra, Metzger, Jaehde, Kalayda: "Cisplatin Protein Binding Partners and Their Relevance for Platinum Drug Sensitivity." in: Cells, Vol. 9, Issue 6, (2020) (PubMed).

Bahraoui, Serrero, Planès: "HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway." in: Scientific reports, Vol. 10, Issue 1, pp. 8177, (2020) (PubMed).

Chen, Wu, Ho, Gung, Tsai, Orekhov, Sobenin, Lin, Yet: "Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration." in: International journal of molecular sciences, Vol. 21, Issue 10, (2020) (PubMed).

Baumann, Hägele, Mochayedi, Drebant, Vent, Blobner, Noll, Nickel, Schumacher, Boos, Daniel, Wendler, Volkmar, Strobel, Offringa: "Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy." in: Nature communications, Vol. 11, Issue 1, pp. 2176, (2020) (PubMed).

Yang, Hsieh, Du, Yen, Hung: "Sequential Interferon β-Cisplatin Treatment Enhances the Surface Exposure of Calreticulin in Cancer Cells via an Interferon Regulatory Factor 1-Dependent Manner." in: Biomolecules, Vol. 10, Issue 4, (2020) (PubMed).

Wang, Yang, Liu, Chun-Kit Tong, Zhu, Malampati, Gopalkrishnashetty Sreenivasmurthy, Cheung, Iyaswamy, Su, Lu, Song, Li: "A Curcumin Derivative Activates TFEB and Protects Against Parkinsonian Neurotoxicity inVitro." in: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).

Liu, Chen, Lii, Jhuang, Huang, Li, Yao: "A Diterpenoid, 14-Deoxy-11, 12-Didehydroandrographolide, in Andrographis paniculata Reduces Steatohepatitis and Liver Injury in Mice Fed a High-Fat and High-Cholesterol Diet." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Loh, Tsai, Huang, Yu, Kuo, Chao, Chou, Tsai, Lee: "Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †." in: Cancers, Vol. 12, Issue 2, (2020) (PubMed).

Yang, Zhu, Tong, Iyaswamy, Xie, Zhu, Sreenivasmurthy, Senthilkumar, Cheung, Song, Zhang, Li: "A stress response p38 MAP kinase inhibitor SB202190 promoted TFEB/TFE3-dependent autophagy and lysosomal biogenesis independent of p38." in: Redox biology, Vol. 32, pp. 101445, (2020) (PubMed).

Chang, Chu, Tsai, Lai, Chen: "Dopamine D3 receptor and GSK3β signaling mediate deficits in novel object recognition memory within dopamine transporter knockdown mice." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 16, (2020) (PubMed).

Wang, Wang, Lin, Kao, Hung, Teng, Tsai, Chi: "Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis." in: Aging cell, Vol. 19, Issue 2, pp. e13090, (2020) (PubMed).

Huang, Chyuan, Shiue, Yu, Hsu, Hsu: "Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade." in: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1822-1836, (2020) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: glyceraldehyde-3-phosphate dehydrogenase (GAPDH Produkte)

Synonyme: G3PD antikoerper, GAPD antikoerper, Gapd antikoerper, BEST:GH12586 antikoerper, CG12055 antikoerper, Dmel\\CG12055 antikoerper, GA3PDH antikoerper, GADPH antikoerper, GAP antikoerper, GAPDH antikoerper, GAPDH I antikoerper, GAPDH-1 antikoerper, GAPDH1 antikoerper, GAPDHI antikoerper, Gapdh antikoerper, Gapdh-1 antikoerper, Gapdh43E antikoerper, gadph antikoerper, gapdh antikoerper, gapdh-1 antikoerper, gh12586 antikoerper, cb609 antikoerper, gapd antikoerper, mg:bb02e05 antikoerper, wu:fb33a10 antikoerper, wu:ft80f05 antikoerper, KNC-NDS6 antikoerper, g3pd antikoerper, G3PDH antikoerper, glyceraldehyde-3-phosphate dehydrogenase antikoerper, Glyceraldehyde 3 phosphate dehydrogenase 1 antikoerper, glyceraldehyde-3-phosphate dehydrogenase S homeolog antikoerper, glyceraldehyde-3-phosphate dehydrogenase, type I antikoerper, olfactory receptor 8K3 antikoerper, GAPDH antikoerper, Gapdh antikoerper, Gapdh1 antikoerper, gapdh antikoerper, gapdh.S antikoerper, gapDH antikoerper, LOC100404960 antikoerper, LOC614985 antikoerper

Hintergrund: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

Cellular Localization: Cytoplasm , perinuclear region , Membrane

Molekulargewicht: 36 kDa

Gen-ID: 2597

UniProt: P04406