VRN2 Antikörper

Produktnummer ABIN7477853

Dieser Maus Monoklonal Antikörper detektiert spezifisch VRN2 in ELISA und WB. Es zeigt Reaktivität gegenüber Proben von Triticum aestivum.

Kurzübersicht für VRN2 Antikörper (ABIN7477853)

Target: VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Reaktivität: Triticum aestivum

Wirt: Maus

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-VRN2 Antikörper

Verwendungszweck: Anti-VRN2 | Vernalization protein 2

Kreuzreaktivität (Details): Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Triticum monococcum

Aufreinigung: Protein G purified

Immunogen: KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Anwendungsinformationen

Applikationshinweise: 1 : 10 000 (ELISA), 1 : 1000 (WB)

Kommentare:

there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Total IgG. Protein G purified purified from Cell culture supernatant.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu VRN2

Target: VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Andere Bezeichnung: VRN2

Hintergrund:

Background: Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Additional information: VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Molekulargewicht: 23.7 kDa