The splenic lymphocytes from BALB/c mouse, immunized with CML-KLH were fused to myeloma P3U1 cells. The cell line (CMS-10) with positive reaction wasgrown in ascitic fluid of BALB/c mouse, from which the antibody was purified byProtein G affinity chromatography and conjugated.
Reaction of protein amino groups with glucose leads, through the early products such as a Schiff baseand Amadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recent immunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modifiedproteins in several human tissues: human lens (nondiabetic and noncataractous), renal proximaltubules in patients with diabetic nephropathy and chronic renal failure, diabetic retina, peripheral nerves of diabetic neuropathy, atherosclerotic lesions of arterial walls, beta2-microglobulin forming amyloid fibrils in patients with hemodialysis-related amyloidosis, senileplaques of patients with Alzheimer