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HES4 Antikörper (AA 94-221)

HES4 Reaktivität: Human WB, ELISA, ICC Wirt: Schaf Polyclonal unconjugated
Produktnummer ABIN291868
  • Target Alle HES4 Antikörper anzeigen
    HES4 (Hairy and Enhancer of Split 4 (HES4))
    Bindungsspezifität
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 94-221
    Reaktivität
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 5
    • 2
    • 1
    • 1
    Schaf
    Klonalität
    • 9
    Polyklonal
    Konjugat
    • 8
    • 1
    Dieser HES4 Antikörper ist unkonjugiert
    Applikation
    • 8
    • 4
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    Spezifität
    Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
    Aufreinigung
    Affinity purified
    Immunogen
    E. coli-derived rhHES-4 (aa94-221).
    Isotyp
    IgG
    Top Product
    Discover our top product HES4 Primärantikörper
  • Applikationshinweise
    Approved: ELISA, ICC, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    deionized water
    Konzentration
    Lot specific
    Buffer
    Lyophilized.
  • Target
    HES4 (Hairy and Enhancer of Split 4 (HES4))
    Andere Bezeichnung
    HES4 (HES4 Produkte)
    Hintergrund
    Name/Gene ID: HES4

    Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4
    Gen-ID
    57801
    UniProt
    Q9HCC6
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