LPIN3 Antikörper (AA 428-529)

Produktnummer ABIN294094

Produktdetails anti-LPIN3 Antikörper

Target: LPIN3 (Lipin 3 (LPIN3))

Bindungsspezifität: AA 428-529

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser LPIN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Spezifität: Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50 % cross-reactivity with rhLipin 3 and less than 5 % cross-reactivity with rhLipin 1 and rhLipin 2.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived recombinant mouse Lipin 3 (aa428-529).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4 % PFA + 14 % picric acid in PBS at 4?C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Details zu LPIN3

Target: LPIN3 (Lipin 3 (LPIN3))

Andere Bezeichnung: LPIN3 / LIPIN-3 (LPIN3 Produkte)

Synonyme: MGC82840 antikoerper, LPIN3 antikoerper, DKFZp469D075 antikoerper, LIPN3L antikoerper, SMP2 antikoerper, dJ450M14.2 antikoerper, dJ450M14.3 antikoerper, dJ620E11.2 antikoerper, 9130206L11Rik antikoerper, AA438110 antikoerper, AV236139 antikoerper, mKIAA4023 antikoerper, lipin 3 L homeolog antikoerper, lipin 3 antikoerper, lpin3.L antikoerper, LPIN3 antikoerper, LOAG_08747 antikoerper, lpin3 antikoerper, Lpin3 antikoerper

Hintergrund: Name/Gene ID: LPIN3

Synonyms: LPIN3, DJ620E11.2, DJ450M14.2, Lipin-3, Lipin 3, Lipin 3-like, Lipin-3-like, SMP2, DJ450M14.3, LIPN3L

Gen-ID: 64900