Keratin Acidic (AE1) Antikörper

Produktnummer ABIN2967160

Produktdetails

Target: Keratin Acidic (AE1)

Reaktivität: Human, Ratte, Huhn, Maus, Kaninchen, Hund, Cat, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.

Kreuzreaktivität (Details): Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Human epidermal keratins

Klon: AE1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Antigendetails

Target: Keratin Acidic (AE1)

Andere Bezeichnung: Acidic Cytokeratins (AE1 Produkte)

Hintergrund: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,Type I cytokeratins