GST
Reaktivität: Diverse Spezies
WB, IP, DB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 1:1000-1:2000, IF: 1: 500, IP: 1:100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid freeze / thaw cycles
Lagerung
-20 °C / -80 °C
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Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.