TUBG1 Antikörper (AA 434-449)

Produktnummer ABIN302067

Produktdetails anti-TUBG1 Antikörper

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Bindungsspezifität: AA 434-449

Reaktivität: Pflanzen, Protozoa

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TUBG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Spezifität: The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.

Kreuzreaktivität (Details): Animals, Protozoa, Plants

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449

Klon: TU-32

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
Immunocytochemistry: Methanol/acetone fixation required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-TUBG1 Antikörper (ABIN302067)

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

Katsetos, Reddy, Dráberová, Smejkalová, Del Valle, Ashraf, Tadevosyan, Yelin, Maraziotis, Mishra, Mörk, Legido, Nissanov, Baas, de Chadarévian, Dráber: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, (2006) (PubMed).

Sulimenko, Dráberová, Sulimenko, Macurek, Richterová, Dráber, Dráber: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Hozák, Dráber: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, (2004) (PubMed).

Linhartová, Novotná, Sulimenko, Dráberová, Dráber: "Gamma-tubulin in chicken erythrocytes: changes in localization during cell differentiation and characterization of cytoplasmic complexes." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 223, Issue 2, pp. 229-40, (2002) (PubMed).

Sulimenko, Sulimenko, Poznanovic, Nechiporuk-Zloy, Böhm, Macurek, Unger, Dráber: "Association of brain gamma-tubulins with alpha beta-tubulin dimers." in: The Biochemical journal, Vol. 365, Issue Pt 3, pp. 889-95, (2002) (PubMed).

Dráberová, Mac?rek, Richterová, Böhm, Dráber: "Monoclonal antibody KN-01 against the heavy chain of kinesin." in: Folia biologica, Vol. 48, Issue 2, pp. 77-9, (2002) (PubMed).

Nohýnkova, Dráber, Reischig, Kulda: "Localization of gamma-tubulin in interphase and mitotic cells of a unicellular eukaryote, Giardia intestinalis." in: European journal of cell biology, Vol. 79, Issue 6, pp. 438-45, (2000) (PubMed).

Binarová, Cenklová, Hause, Kubátová, Lysák, Dolezel, Bögre, Dráber: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, (2000) (PubMed).

Dráberová, Dráberová, Surviladze, Dráber, Dráber: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, (1999) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Details zu TUBG1

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Andere Bezeichnung: gamma-tubulin (TUBG1 Produkte)

Synonyme: GCP-1 antikoerper, TUBG antikoerper, TUBGCP1 antikoerper, TUBC antikoerper, TUBG3 antikoerper, gamma-tubulin antikoerper, Tubg2 antikoerper, xgam antikoerper, 1500010O08Rik antikoerper, AI451582 antikoerper, AI503389 antikoerper, Tubg antikoerper, tubgl antikoerper, zgc:55894 antikoerper, tubulin gamma 1 antikoerper, gamma-tubulin 1 antikoerper, gamma-tubulin antikoerper, tubulin gamma 1 L homeolog antikoerper, Tubulin gamma chain antikoerper, tubulin, gamma 1 antikoerper, tubulin gamma-1 chain antikoerper, TUBG1 antikoerper, tubg1 antikoerper, tubg1.L antikoerper, tbg-1 antikoerper, Tubg1 antikoerper, LOC101686824 antikoerper, LOC101103906 antikoerper

Hintergrund: Tubulin gamma 1,The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.,TUBG

Gen-ID: 7283

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase