APP Antikörper (AA 190-290)

Produktnummer ABIN3020680

Produktdetails anti-APP Antikörper

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Bindungsspezifität: AA 190-290

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: AEESDNVDSA DAEEDDSDVW WGGADTDYAD GSEDKVVEVA EEEEVAEVEE EEADDDEDDE DGDEVEEEAE EPYEEATERT TSIATTTTTT TESVEEVVRE V

Kreuzreaktivität: Human

Produktmerkmale: Polyclonal Antibodies

Aufreinigung: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 190-290 of human APP (NP_000475.1).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #104427 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104427

Datum: 12.05.2022

Antigen: APP

Chargennummer: 3100080101

Validierte Anwendung: Immunohistochemistry

Positivkontrolle:

Adult (24 months) mouse brain

3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

Negativkontrolle:

We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

Bewertung:

The anti-APP antibody (Amyloid beta (A4) Precursor Protein) C-Term ABIN3020680 works in IHC-P, especially at higher concentrations (1:50 and 1:100), and without the use of any antigen retrieval treatment.

Validierungsbilder

Staining of beta-amyloid positive cells in the adult mouse brain using ABIN3020680 at 40x magnification.

Protokoll

Primärantikörper: ABIN3020680

Sekundärantikörper: goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)

Full Protocol:

• Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
• Wash, dehydrate, and embed samples in paraffin wax.
• Wash several times with 0.01 M PBS.
• Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
• After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
• Incubate sections with primary anti-APP antibody (Amyloid beta (A4) Precursor Protein) (N-Term) (antibodies Online, ABIN3020680, lot 3100080101) diluted 1:50, 1:100, and 1:200 in PBS-BSA-PLL ON at RT.
• Wash sections 3x 5 min with 0.01 M PBS.
• Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
• Wash sections 3x 5 min with 0.01M PBS.
• Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
• Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.

Anmerkungen:

Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min (95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Andere Bezeichnung: APP (APP Produkte)

Synonyme: AAA antikoerper, ABETA antikoerper, ABPP antikoerper, AD1 antikoerper, APPI antikoerper, CTFgamma antikoerper, CVAP antikoerper, PN-II antikoerper, PN2 antikoerper, aaa antikoerper, abeta antikoerper, abpp antikoerper, ad1 antikoerper, appi antikoerper, ctfgamma antikoerper, cvap antikoerper, pn2 antikoerper, APP antikoerper, APP-like antikoerper, APPL antikoerper, Abeta antikoerper, BcDNA:GH04413 antikoerper, CG7727 antikoerper, Dmel\\CG7727 antikoerper, EG:65F1.5 antikoerper, appl antikoerper, Abpp antikoerper, Adap antikoerper, Ag antikoerper, Cvap antikoerper, E030013M08Rik antikoerper, betaApp antikoerper, app antikoerper, wu:fj34d10 antikoerper, wu:fk65e12 antikoerper, zgc:85740 antikoerper, amyloid beta precursor protein antikoerper, amyloid beta (A4) precursor protein antikoerper, beta amyloid protein precursor-like antikoerper, amyloid beta (A4) precursor protein a antikoerper, amyloid beta precursor protein L homeolog antikoerper, APP antikoerper, app antikoerper, Appl antikoerper, App antikoerper, appa antikoerper, app.L antikoerper

Hintergrund: This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.,AAA,ABETA,ABPP,AD1,APPI,CTFgamma,CVAP,PN-II,PN2,Amyloid beta A4,APP,Aβ42,preA4,Epigenetics & Nuclear Signaling,Transcription Factors,Signal Transduction,G protein signaling,Cell Biology & Developmental Biology,Apoptosis,Immunology & Inflammation,IL-6 Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,Neurodegenerative Diseases Markers,APP

Molekulargewicht: 34 kDa/72-86 kDa

Gen-ID: 351

UniProt: P05067

Pathways: Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour