Keratin 10/13 Antikörper

Produktnummer ABIN302093

Produktdetails

Target: Keratin 10/13

Reaktivität: Human, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

Kreuzreaktivität (Details): Human, Feline (Cat), Other species Not tested

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.

Klon: DE-K13

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/mL (1:100), positive tissue: tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH . The antibody DE-K13 reacts exclusively with Cytokeratin 13.
Immunohistochemistry (frozen sections): Use at an assay dependent dilution.
Western blotting: recommended dilution: 1-3 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Kozaki, Nakamura, Iguchi, Kano, Watanabe, Fujiwara, Hasegawa: "Immunohistochemical analysis of cytokeratin expression in dog skin." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 1, pp. 1-4, (2001) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard, Misdorp: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." in: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).

Antigendetails

Target: Keratin 10/13

Andere Bezeichnung: Cytokeratin 10+13

Hintergrund: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).,K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13