AHNAK Antikörper (N-Term)

Produktnummer ABIN302097

Produktdetails anti-AHNAK Antikörper

Target: AHNAK (AHNAK Nucleoprotein (AHNAK))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AHNAK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Kreuzreaktivität (Details): Human, Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Klon: EM-09

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Positive control: HeLa human cervix carcinoma cell line.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-AHNAK Antikörper (ABIN302097)

Johnson, Zhang, Adams, Phillips, Freitas, Froehner, Green-Church, Montanaro: "Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins." in: PLoS ONE, Vol. 7, Issue 8, pp. e43515, (2012) (PubMed).

Details zu AHNAK

Target: AHNAK (AHNAK Nucleoprotein (AHNAK))

Andere Bezeichnung: AHNAK1 (AHNAK Produkte)

Synonyme: AHNAKRS antikoerper, 1110004P15Rik antikoerper, 2310047C17Rik antikoerper, AA589382 antikoerper, AV091586 antikoerper, DY6 antikoerper, AHNAK 1 antikoerper, RGD1308572 antikoerper, UV118 antikoerper, AHNAK antikoerper, wu:fb05a01 antikoerper, wu:fu74f12 antikoerper, LOC733715 antikoerper, AHNAK nucleoprotein antikoerper, AHNAK nucleoprotein (desmoyokin) antikoerper, AHNAK nucleoprotein pseudogene antikoerper, AHNAK antikoerper, Ahnak antikoerper, ahnak antikoerper, LOC748622 antikoerper

Hintergrund: AHNAK1 nucleoprotein,AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear, it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.,Desmoyokin

Gen-ID: 79026

UniProt: Q09666