Clathrin Heavy Chain (CLTC) Antikörper

Produktnummer ABIN302098

Produktdetails

Target: Clathrin Heavy Chain (CLTC)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.

Kreuzreaktivität (Details): Human, Porcine, Mouse, Rat, Bovine, Other not determined

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Klon: BF-06

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 2-5 μg/mL, positive control: human blood leukocytes. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Mac?rek, Zíková, Dráberová, Dráber: "Monoclonal antibody BF-06 against the heavy chain of clathrin." in: Folia biologica, Vol. 49, Issue 6, pp. 238-40, (2004) (PubMed).

Antigendetails

Target: Clathrin Heavy Chain (CLTC)

Andere Bezeichnung: Clathrin heavy chain (CLTC Produkte)

Hintergrund: Clathrin heavy chain,Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.,CHC, CLHC, MRD56

Gen-ID: 1213

UniProt: Q00610