GTPase NRas Antikörper (Internal Region)

Produktnummer ABIN302138

Produktdetails anti-GTPase NRas Antikörper

Target: GTPase NRas (NRAS)

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GTPase NRas Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: NRAS

Sequenz: RMKKLNSSDD GTQ

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GTPase NRas

Target: GTPase NRas (NRAS)

Andere Bezeichnung: NRAS (NRAS Produkte)

Synonyme: NRAS antikoerper, AV095280 antikoerper, N-ras antikoerper, ALPS4 antikoerper, NRAS1 antikoerper, NS6 antikoerper, N-RAS antikoerper, fj99a10 antikoerper, wu:fj99a10 antikoerper, zgc:100761 antikoerper, zras-b1 antikoerper, H-RAS antikoerper, HRAS antikoerper, GTPase NRas antikoerper, neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog antikoerper, NRAS proto-oncogene, GTPase antikoerper, neuroblastoma ras oncogene antikoerper, neuroblastoma RAS viral oncogene homolog antikoerper, Tsp_01117 antikoerper, nras.L antikoerper, Nras antikoerper, NRAS antikoerper, nras antikoerper

Hintergrund: NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog

Gen-ID: 4893, 18176, 24605

NCBI Accession: NP_002515

Pathways: p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling