mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
Aufreinigung
Protein G affinity chromatography
Immunogen
The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
HepPar-1
Reaktivität: Human
IF, ICC
Wirt: Maus
Monoclonal
HSA133
CF®594
Applikationshinweise
The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.\. Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed cells): 0.5-1 μg/mL for 30 minutes at RT
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Konservierungsmittel
Azide free
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store the Hepatocyte Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.